By Walker J.M. (ed.), Pollard J.W. (ed.)
CIP cites the sequence because the major access. Covers equipment for developing mammalian fibroblastic mobilephone cultures and retaining tradition stipulations for epithelial, neuronal, and hematopoietic cells between others. realization is given to the range of tradition media and extracellular matrices had to retain the differential capabilities of the aesthetic cells. additionally describes tradition concepts for either dwelling and glued cells.
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Extra info for Animal Cell Culture
Develop0Bid. 55,81-92. B. ), IRL Press,Oxford, pp. 33-70. Griffiths, J. , Cameron, D. , and Looby, D. (1987) A comparison of unit process systems for anchorage dependent cells. Develop. Bid. Sfunduyd,66,331-338. Griffiths, J. B. (1986) Can cell culture medium costsbe reduced? Strategies and possibilities. 4,268-272. P. and Spier, R. E. l to lOOliter of aunit processsystembased on 3 mm diameter glass spheres for the production of four strains of FMDV from BHK monolayer cells. 23,551-565. Chapter Mycoplasma 7 Detection J.
Microcarrier culture is not a difficult technique, but it does require more critical attention to experimental detail than most methods and the use of the correct culture vessels. 3 (Pfeifer & Langen) at 3 g/L. Prepare the microcarriers according to manufacturer’s recommendations. 1. It is essential that the medium with microcarriers be prewarmed and stabilized before inoculating the cells. Cell attachment to moving spheres requires conditions to be just right. , have not been standing in trypsin, and so on, for extended periods).
J. Immunol. 5,45-55. Hudson, L. and Hay, F. C. (1980) Practical Immunology (Blackwell Scientific Publications), pp. 212,213. , and Sjoquist, J, I. (1978) Separation of cells by affinity chromatography on SPA-Sepharose 6MB. J. Immunol. Methods 21,133-141. , Rossio, J. , Ruscetti, F. , and Oppenheim, J. J. (1986) Establishment of a human B cell line that proliferates in response to B cell growth factor. J. Immunol. Methods 90,111-123. Chapter 5 Establishment of Lymphoblastoid Cell Lines A. Doyle 1.
Animal Cell Culture by Walker J.M. (ed.), Pollard J.W. (ed.)