Download e-book for iPad: Cell-Cell Interactions: Methods and Protocols (Methods in by Sean P. Colgan

By Sean P. Colgan

ISBN-10: 1588295230

ISBN-13: 9781588295231

ISBN-10: 1597451134

ISBN-13: 9781597451130

A flexible choice of easily reproducible cell-cell interplay assays for uncovering mobile interactions on the molecular point, either in vitro and in vivo. The protocols disguise a various set of cell-cell interplay versions in either basic and pathological states, are conveniently adaptable to almost any telephone sort and organ approach, and comprise basic facts and final result research. furthermore, the protocols stick to the winning tools in Molecular Biology™ sequence layout, every one supplying step by step laboratory directions, an creation outlining the foundations in the back of the strategy, lists of the mandatory apparatus and reagents, and tips about troubleshooting and heading off identified pitfalls.

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Physiol. 48, 241–250. 43. Miller, D. , Nobmann, S. , and Fricker, G. (2000) Xenobiotic transport across isolated brain microvessels studied by confocal microscopy. Mol. Pharmacol. 58, 1357–1367. 44. Goldstein, G. , Wolinsky, J. , and Diamond, I. (1975) Isolation of metabolically active capillaries from rat brain. J. Neurochem. 25, 715–717. 2 Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells Methods to Characterize Microflora and Functional Consequences Geraldine Canny, Alexander Swidsinski, and Beth A.

32 Canny, Swidsinski, and McCormick Thereafter, allow the plate to warm to 37°C, add the bacterial suspension and continue with the procedure above. Additional controls should be included to rule out the possibility of the antibody affecting bacterial growth. 4. Notes 1. Initial growth curves should be conducted to relate absorbance to bacterial number. We have used this protocol to assess the contribution of BPI to bacterial killing mediated by intestinal epithelial cells but the assay can be adapted for other mucosal epithelial cells using a relevant strain of bacteria.

Med. Microbiol. 30, 149–156. 8. MacDermott, R. P. (1999) Chemokines in the inflammatory bowel diseases. J. Clin. Immunol. 19, 266–272. 9. Ajuebor, M. N. and Swain, M. G. (2002) Role of chemokines and chemokine receptors in the gastrointestinal tract. Immunology 105, 137–143. 10. Pavlick, K. , Laroux, F. , et al. (2002) Role of reactive metabolites of oxygen and nitrogen in inflammatory bowel disease (1,2). Free Radic. Biol. Med. 33, 311–322. 11. , and Madara, J. L. (1987) Effects of polymorphonuclear leukocyte transmigration on the barrier function of cultured intestinal epithelial monolayers.

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Cell-Cell Interactions: Methods and Protocols (Methods in Molecular Biology Vol 341) by Sean P. Colgan


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