By MM. Srivastava (auth.), ManMohan Srivastava (eds.)
The e-book is equipped in 4 chapters comprising of 18 contributions from specialists of foreign reputation. The booklet highlights HPTLC procedure as a flexible glossy analytical procedure almost about its very good automation, optimization, multidimensional purposes. It offers with basics, precept, thought of HPTLC established separation, experimental info, dealing with figuring out and implementation. It describes a variety of purposes of HPTLC separation with specific connection with phytochemical research, fingerprint research, authentication, biomedical research, drug quantification, analytical research, quantitative research and validation. HPTLC and its destiny in combinatorial strategy provides new size for its capability for hyphenation, HPTLC-MS coupling, laser desorption-HPTLC chromatography coupled with chemical ionization mass spectrometry etc.
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Extra resources for High-Performance Thin-Layer Chromatography (HPTLC)
R. H. R. Patel Indukaka Ipcowala College of Pharmacy, Sardar Patel University, New Vallabh Vidyanagar 388121, Gujarat, India MM. B. Patel et al. is a powerful analytical method equally suitable for qualitative and quantitative analytical tasks. This has been demonstrated by many publications and research projects. HPTLC is a flexible, versatile and economical process in which the various stages are carried out independently. The advantages of this off-line arrangement as compared with an on-line process, such as column high-performance liquid chromatography (HPLC), have been outlined and include the following (Sherma and Fried 1996; Sethi 1996; Sherma 2007): l l l l l l l l l l l l l l l l Technically, it is simple to learn and operate.
Third level involves trying of mixtures instead of neat solvents from the selected solvents of first and second level which can further be optimized by the use of modifier like acids or bases. Analytes are detected using fluorescence mode or absorbance mode. But, if the analytes are not detected perfectly than it needs change of stationary phase or mobile phase or need the help of pre or post chromatographic derivatization. Optimization can be started only after a reasonable chromatogram which can be done by slight change in mobile-phase composition.
This leads to a reasonable chromatogram which has all the desired peaks in symmetry and well separated. Procedure for HPTLC method development is outlined as follow (Figure 1). (Sherma and Fried 1996; Sethi 1996). B. Patel et al. SCHEMATIC PROCEDURE FOR HPTLC METHOD DEVELOPMENT Sample and standard preparation Selection of chromatographic layer Layer pre - washing Linomat 5 Applicator Layer pre - conditioning Application of sample and standard Chromatographic development Detection of spots, scanning and documentation Twin trough Chamber Scanner Photo documentation with digital camera 3 Experimental Aspects and Implementation of HPTLC 45 Stationary Phase HPTLC can be regarded as the most advanced form of modern TLC.
High-Performance Thin-Layer Chromatography (HPTLC) by MM. Srivastava (auth.), ManMohan Srivastava (eds.)