New PDF release: Microbiology of Composting

By J. N. Cooper, J. G. Anderson, C. D. Campbell (auth.), Univ. Doz. Dr. Heribert Insam, Nuntavun Riddech M. Sc., Mag. Susanne Klammer (eds.)

ISBN-10: 3642087051

ISBN-13: 9783642087059

ISBN-10: 3662087243

ISBN-13: 9783662087244

Composting is more and more used as a recycling know-how for natural wastes. wisdom at the composition and actions of compost microbial groups has to this point been in response to conventional equipment. New molecular and physiological instruments now provide new insights into the "black field" of decaying fabric. An unexpected range of microorganisms are fascinated by composting, commencing up an immense capability for destiny technique and product advancements. during this booklet, the perspectives of scientists, engineers and end-users on compost construction, approach optimisation, standardisation and product software are presented.

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Additional resources for Microbiology of Composting

Sample text

The rDNA fragment amplified from compost DNA was cloned into the pGEM-T vector (Promega, Madison, WI). Competent cells of E. coli were transformed with the ligated DNA and then plated on LB plates contammg ampicillin and X-Gal (5-bromo-4-chloro-3-indolyl-b-Dgalactopyranoside). White E. coli colonies, presumably carrying pGEM-T with an rDNA insert, were transferred to 96-well microtiter plates containing LBampicillin and cultured. The rDNA inserts from individual clones in the library were amplified with the SP6 and T7 primers and directly sequenced using the T7 primer and an automated ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, CA).

2002). The purified DNA extract solutions were colorless, indicating that they were free of humic acid contamination. Agarose gel electrophoresis indicated that the aproxirnate DNA yield was 6 Ilg g-' compost and that it had an MW greater than 20 kb. 165 rRNA Gene Amplification and Digestion Ribosomal 16S rRNA genes were PCR amplified from purified DNA samples using universal eubacterial primers 8F hex (fluorescently labeled forward primer) and 1392R (reverse primer). The sequences (5'-3') of the forward and reverse primers were: AGAGTTTGATCCTGGCTCAG and ACGGGCGGTGTGTRC, respectively (Liu et al.

In: Van Griensven LJLD (ed) Mushroom science XV Science and cultivation of edible fungi. Balkema, Rotterdam, pp 401-407 Keohavong P, Thilly WG (1989) Fidelity of DNA polymerases in DNA amplification. Proc Natl Acad Sci USA 86: 9253-9257 Koschinsky S, Schwieger F, Peters S, Grabbe K, Tebbe CC (1998) Characterizing microbial communities of composts at the DNA level. Med Fac Landbouww Univ Gent 6314b: 1725-1732 O'Donnell K, Cigelnik E, Weber NS, Trappe JM (1997) Phylogenetic relationships among ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis.

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Microbiology of Composting by J. N. Cooper, J. G. Anderson, C. D. Campbell (auth.), Univ. Doz. Dr. Heribert Insam, Nuntavun Riddech M. Sc., Mag. Susanne Klammer (eds.)

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