By Michael F. Tuite, Stephen G. Oliver
In this quantity we goal to offer an easy-to-read account of the genus Saccharomyces that we are hoping can be of worth to all scholars and researchers wishing to take advantage of this significant genus, be it for tutorial or commer cial reasons. person chapters were commissioned to hide particular features of the biology of Saccharomyces species: development, genetics, and metabolism, with the emphasis on method. easy rules are mentioned with no an over-detailed, step by step breakdown of particular thoughts, and long discussions of ordinary molecular, organic, and biochemical strategies (e. g. , polyacrylamide gel electrophoresis, protein purification, DNA sequencing) were kept away from. we are hoping the amount will supply a short connection with the present prestige of a variety of Saccharomyces-specific methodologies with no focusing ex clusively on contemporary advancements in molecular strategies that are present in the ever expanding numbers of "cloning manuals. " by means of necessity, a lot of what's defined during this quantity concentrates on one specific species of Saccharomyces, particularly Saccharomyces cerevisiae. this isn't only a mirrored image of the authors' pursuits, yet shows the level to which this straightforward eukaryote has been studied through biologists from all walks of lifestyles, for all types of purposes. If this quantity provides a broader wisdom base to the skilled yeast researcher, or ease the trail of somebody simply beginning paintings with Saccharomyces, then we'll have completed our aim.
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Additional resources for Saccharomyces
The efficiency of different lipid extraction methods suitable for yeast cells has been compared. Initial heating in 80% ethanol has been recommended for complete extraction of polar lipids and inactivation of lipases (Hanson and Lester, 1980). An array of glycerophospholipids typical for eukaryotic cells is found 17 18 C. KREUTZFELDT and W. WITT in the group of the polar lipids, comprising phosphatidylcholine, -ethanolamine, -monomethylethanolamine, dimethylethanolamine, -glycerol and -inositol, cardiolipin, phosphatidic acid, and lyso compounds.
2. Cell Wall The cell wall structure and the synthesis and assembly of its constituents as a simple and versatile model for morphogenesis have been studied intensively. , tunicamycin); and electron microscopic procedures. , 1984; Catley, 1983). Glucan and mannoprotein, the main structural constituents of the S. siae cell wall, are found in roughly equal amounts. , 1983). , 1975, for review and references). , 1982; Gatley, 1983). The shape of(~ 1-3)glucan has been described as a long, twisted chain with a hydrophilic side due to hydroxyl groups and a hydrophobic side due to methine groups.
KREUTZFELDT and W. WITT separation are not perfect and that contamination from organelle membranes cannot be excluded, leading to erroneous results in respect to subcellular distribution of enzyme activities. One of the reasons is that the endoplasmic reticulum is closely associated with the plasma membrane and connections between both membranes may exist (Schekman and Novick, 1982). Plasma membrane fractions isolated by different procedures may vary greatly in protein and lipid composition (see Section 5).
Saccharomyces by Michael F. Tuite, Stephen G. Oliver